DNA Oligonucleotides
Consistently generate reliable data from high-quality oligos
Ordering
- Complete confidence in oligos that are checked by electrospray ionization mass spectrometry (ESI-MS)*
- Begin your project sooner with >90% of orders shipped within 24 hours†
- Extensive control of oligo specifications with custom formulation and mixing options
*With the exception of mixed base oligos, which could potentially represent multiple sequences and therefore cannot be evaluated by ESI-MS.
†Small scale, standard, desalted oligos
Single-stranded DNA
Shipped dry, or resuspended to your specifications.
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Normalized Oligo NEW
Custom normalized oligos up to 10 grams
| Product | Length |
|---|---|
| Normalized oligo | 5-80 bases |
RxnReady™ Primer Pools
2 oligos premixed in a single tube. Shipped dry, or resuspended to your specifications.
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The following mixing fee will be applied to each pool:
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Duplexed DNA
2 oligos, annealed and delivered in a single tube. Shipped dry.
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The following annealing fees will be applied to each duplex ordered:
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Single-stranded DNA
Shipped dry, or resuspended to your specifications. A minimum of 24 and 96 oligos required for 96- and 384-well plates, respectively. 500 pmol scale requires a minimum of 96 oligos.
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Primer mix DNA
2 premixed oligos per well. Shipped dry or resuspended to your specifications. A minimum of 24 and 96 mixes is required for 96- and 384-well plates, respectively.
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For ordering inquiries, please contact us.
Duplexed DNA
1 oligo pair per well, annealed. Shipped dry. A minimum of 24 and 96 oligo duplexes are required for 96- and 384-well plates, respectively.
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The following annealing fee will be applied to each plate of duplexed DNA:
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For ordering inquiries, please contact us.
SameDay™ Oligos
Order today by the geographical deadline and your oligos will ship same business day. Shipped dry, or resuspended to 100 µM in IDTE buffer, pH 8.0. Learn more »
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HOTPlates™ Oligo Service
Order today and your oligos will ship the following business day. Delivered in 96-well, V-bottom plates. A minimum of 48 oligos is required per plate. Shipped dry. Learn more »
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The following service fee will be added to each plate:
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Rapid HPLC™ Oligos
Order today and your oligos will ship the following business day. Shipped dry in tubes. Learn more »
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Request a consultation
Not seeing what you need? Reach out to us! Our team is here to handle almost any request for custom oligos and support your research goals.
Request a consultationProduct Details
DNA Oligonucleotides are short, single- and double-stranded synthetic DNA sequences that can be used in a variety of molecular biology applications. Using the Oligo Entry ordering tool, you can design your oligos to contain any DNA sequence you require and select from hundreds of modifications to suit your specific research needs.
Every DNA Oligo you receive will be deprotected and desalted to remove small molecule impurities. In addition, your oligos will be quality controlled via proprietary ESI-MS
* With the exception of mixed base oligos, which could potentially represent multiple sequences and therefore cannot be evaluated by ESI -MS.
For standard, desalted oligos ≥20 bases, we offer the following yield guarantees:
| Product | Available length | Guaranteed yield* |
|---|---|---|
| 25 nmole DNA Oligo | 15–60 bases | 10 nmol |
| 100 nmole DNA Oligo | 10–90 bases | 30 nmol |
| 250 nmole DNA Oligo | 5–100 bases | 50 nmol |
| 1 µmole DNA Oligo | 5–100 bases | 200 nmol |
| 2 µmole DNA Oligo | 5–100 bases | 400 nmol |
5 µmole DNA Oligo | 5–100 bases | 1000 nmol |
| 10 µmole DNA Oligo | 5–100 bases | 2000 nmol |
*Guaranteed yield for unmodified oligos 25–50 bases; will vary with oligo composition, length, and purification.
Product Data
IDT's long-standing reputation as a pioneer in custom oligo manufacturing is largely due to our vertically integrated proprietary synthesis platforms. Nearly every component of our manufacturing process is designed and developed in-house, including specialized synthesizers that can accommodate the most demanding oligo requests, and high-throughput automation systems that ensure fast turnaround times. Our ability to control these variables allows us to consistently produce high-quality oligos for use in routine and specialized applications.
An important measure of quality for any oligo manufacturer is coupling efficiency, because this directly affects the amount of full-length product you receive. Our manufacturing processes result in high coupling efficiencies (Figure 1) and are standardized across our global manufacturing sites (Figure 2) to provide you with most consistent quality in the industry. No matter where in the world you are conducting your research, an IDT oligo is an IDT oligo. Our commitment to quality and consistency results in a reliable product that helps provide confidence in your research.
Figure 1. IDT proprietary platforms have a better coupling efficiency than other suppliers, which provides more full-length oligonucleotides in your order. Small increases in coupling efficiency (≤1%) result in measurable increases in full-length product yield. The curves represent the theoretical yield for different lengths of oligos based on a coupling efficiency of 99.4% (IDT Oligos, n = 126) and 99.1% (other suppliers, n = 134 from three different suppliers) using the formula: percent full length product = (eff )(n-1)*100 where eff = coupling efficiency (for example, 99.4% = 0.994) and (n–1) is the number of coupling reactions needed to make an oligo of length n.
Figure 2. IDT tracks coupling efficiency at our manufacturing sites to ensure consistent quality. Many variables can lead to reduced oligo quality, including ambient temperature, humidity, and input reagents. Monitoring of coupling efficiency at IDT manufacturing sites ensures that we provide our customers with high-quality products. A quarterly average is shown (n = 16 per manufacturing site).
Services
The following services represent our most common offerings. If your project requires a service not listed here, contact us and we will work with you individually to accommodate your request.
Purification
For demanding applications such as multiplex PCR, cloning, mutagenesis, or antisense/RNAi methods, additional purification may be needed for your oligonucleotides. All purified oligos up to 60 bases receive a purity guarantee via QC by capillary electrophoresis. Learn more »
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Formulation
Specific research requirements and individual preferences require oligos that can be delivered in unique formats. With our formulation and packaging services, you can have your oligos manufactured to your specific requirements. Learn more »
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Custom quality control
In addition to the free ESI-MS analysis provided for all oligos, your project may require one of the analytical services listed below. Learn more »
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Large-scale synthesis
Custom DNA Oligos can be ordered in bulk with the same high purity, competitive pricing, and fast turnaround times you expect from IDT. To learn more about our large-scale synthesis capabilities, click here.
GMP and OEM
If you require oligos that are ISO 13485:2016 certified, or if you are interested in our third-party manufacturing services, please click here.
Resources
User guides and protocols
Handbooks
Brochures and flyers
Certificates of analysis (COAs)
Frequently asked questions
What is the difference between the locked nucleic acids in Affinity Plus™ qPCR Probes and PrimeTime™ LNA® qPCR Probes?
There are no structural differences between the locked nucleic acids used in Affinity Plus and PrimeTime LNA* qPCR Probes. Functional data can be found on the Affinity Plus qPCR Probes page. While these modified probes have identical chemical structures and physical properties, Affinity Plus qPCR Probes provide a better value.
Learn more about Affinity Plus qPCR Probes.
*LNA is a registered trademark of Qiagen.
How do I convert my yield from nanomoles to ODs?
Calculating this conversion requires you to know the extinction coefficient for your sequence. With this, you can easily convert from your nanomole amount to ODs. To determine the extinction coefficient, you can analyze your sequence using IDT's free OligoAnalyzer™ Tool. Results from this analysis will also provide you with nmol/OD260 and µg/OD260 values.
GMP refers to products manufactured under ISO 13485: 2016 QMS. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations for their legal marketing.