Alt-R™ CRISPR Custom Guide RNAs
Guide RNAs for novel Cas enzymes (e.g., prime editing or the Cas13 family)
Our well-established expertise in oligo synthesis combined with an active CRISPR research and development program are what make us a trusted vendor for custom gRNA synthesis.
Ordering
CRISPR Custom Guide RNAs
We offer chemically synthesized and modified custom guide RNAs for common as well as specialized research applications. Alt-R Custom Guide RNAs are ideal for prime editing (pegRNA) projects, CRISPR-Cas13 applications, and most alternative CRISPR-Cas systems.
- Fast shipping (usually 3–6 business days)
- A range of modification options
- Multi-scale delivery in tubes or plates
We also offer a complete selection of Cas9 guide RNAs (sgRNA, crRNA, and tracrRNA) as well as Cas12a guide RNAs.
All our CRISPR guide RNAs are available with chemical modifications that increase stability, potency, and/or nuclease resistance. Some modifications can decrease unwanted immune responses in your experiments. Guide RNAs in tubes are available in either HPLC-purified or standard desalt formats.
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Flexibility to meet a wide range of guide RNA needs
Alt -R CRISPR Custom Guide RNAs are chemically synthesized and modified. These guide RNAs include pegRNA for the prime editing system as well as guide RNAs for the Cas13 family of enzymes and other unusual Cas systems.
Prime editing guide RNA (pegRNA)
The laboratory of David Liu (Broad Institute of MIT and Harvard, Cambridge, MA) has published a CRISPR genome editing technique called prime editing [1]. This utilizes a Cas9 H840A nickase fused to a reverse transcriptase, in combination with a long guide RNA (prime editing guide RNA or pegRNA). The pegRNA encodes the new sequence and allows transcription to introduce the desired mutations. This system may have great promise for research geared towards the development of new therapies for genetic diseases. It is an exciting complement to existing CRISPR editing systems and may even be an improvement in many cases.
Cas13 guide RNA
The CRISPR-Cas13 family of enzymes targets and cuts RNA instead of DNA. Papers from the Feng Zhang lab at the Broad Institute and the Stanley Qi lab at Stanford University demonstrate the potential of CRISPR-Cas13 for SARS-CoV-2 identification as well as the research studies geared towards the development of COVID-19 therapies. [2,3]
Resources
Frequently asked questions
What CRISPR nucleases are available from IDT?
We are rapidly expanding our offerings—please check back regularly for additional options.
What purifications are supported through the Alt-R™ CRISPR Custom Guide RNA ordering tool?
Should pegRNAs for CRISPR prime editing include chemical modifications?
How can users include modifications or base types other than what is available in the Alt‑R™ CRISPR Custom Guide RNA ordering tool?
For modifications or base types other than what is available in the Alt-R™ CRISPR Custom Guide RNA ordering tool, please contact us for additional information and recommendations.
What bases and chemical modifications are supported through the Alt-R™ CRISPR Custom Guide RNA ordering tool?
References
- Anzalone AV, Randolph PB, Davis JR, et al. Search-and-replace genome editing without double-strand breaks or donor DNA. Nature. Dec 2019;576(7785):149-157.
- Lin X, Liu Y, Chemparathy A, Pande T, La Russa M, Qi LS. A comprehensive analysis and resource to use CRISPR-Cas13 for broad-spectrum targeting of RNA viruses. Cell Rep Med. 2021;2(4):100245.
- Patchsung M, Jantarug K, Pattama A, et al. Clinical validation of a Cas13-based assay for the detection of SARS-CoV-2 RNA. Nat Biomed Eng. 2020;4(12):1140-1149.