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Targeted DNA sequencing

For nearly 30 years, IDT has been by your side as the global leader in oligonucleotide synthesis. Targeted DNA sequencing extends this commitment across a broad range of custom or ready-made solutions to help you reach the next phase of your research with consistency and reliability.

xGen™ NGS—made to work.

Amplicon sequencing

Amplicon sequencing allows for multiplexing of samples where numerous PCR amplicons are converted into library molecules in an efficient workflow. This method is applicable to a wide range of research areas and can reduce costs and workflow time. It is also useful for the discovery of rare mutations in complex samples and hard to sequence areas.

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Hybridization capture

Hybridization capture targets regions of the genome specific to researchers’ objectives using 5’ biotinylated probes. After isolating probes annealed to complementary fragments with streptavidin-coated beads, the library fragments are sequenced. The resulting data focus on the targeted regions providing more read depth for variant identification.

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Overview

  • Cost-effective—simplified workflows can target regions of interest using capture and enrichment strategies
  • Super amplicons—xGen Amplicon Technology can identify variants that could drop out of other amplicon sequencing workflows
  • Ideal coverage—targeted hybridization capture workflow for on target and uniform coverage that supports single- or multi-plex options

What is targeted DNA sequencing?

Targeted DNA sequencing methods allow for the interrogation of specific regions of the genome so that researchers can obtain information about the genetic material they care about the most. Targeted NGS workflows, such as amplicon sequencing or hybridization capture, generally require less sample input and result in data on specific areas of the genome, meaning that researchers get more manageable datasets for in-depth analyses.

For more information about targeted DNA sequencing read the IDT Targeted sequencing handbook, or read additional information in the following: amplicon sequencing or hybridization capture.

Amplicon workflow

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Extraction

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Library prep

Choose the appropriate amplicon sequencing panel:

xGen Amplicon UDI Primers

xGen Amplicon Core Kit (found on individual amplicon panel pages)

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Sequencing & analysis

IDT ALIGN℠ Program

Hybridization Capture Workflow

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Extraction

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Library Prep

Choose the appropriate xGen NGS DNA Library Prep Kit:

Choose the adapter for your platform and application:
xGen Adapters & Indexing Primers

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Hyb Capture Core Reagents and Panels

Choose the appropriate amplicon sequencing panel:

Add each xGen Hybridization Capture Core Reagents:

  • xGen Hybridization and Wash v2 Kit
  • xGen Universal Blockers TS
  • xGen Library Amplification Primer Mix
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Sequencing & analysis

IDT ALIGN℠ Program

Request a consultation

Our leading NGS experts are ready to assist with your sequencing questions. Let us help you overcome barriers by requesting a consultation today.

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