xGen™ NGS Library Preparation & Adapters
With xGen NGS, we’ve made library preparation easy. Our solutions were developed to improve your time, yields, and budget across a broad range of sample types. Wherever your research journey takes you, you can trust in xGen NGS to assist.
xGen NGS—made easy.
Overview
Library preparation is a necessary step to turn input DNA or RNA into final libraries before targeted capture or sequencing (Figure 1). Our comprehensive catalog of library preparation solutions can help you no matter your research application, such as:
- DNA sequencing (whole genome and targeted sequencing)
- RNA sequencing (whole transcriptome and targeted sequencing)
- Methylation sequencing (whole genome and targeted bisulfite sequencing)
- Chromatin immunoprecipitation (ChIP) sequencing
- Targeted sequencing with hybridization capture
- PCR-free workflows
- Oncology research
- Metagenomics research
All xGen library prep kits are automation friendly. For more information, please contact us.
All xGen library prep kits are compatible with xGen Normalase™ Module. The proprietary
technology is a novel enzymatic library normalization method that eliminates the need for library concentration adjustment prior to sequencing.
Figure 1. Example of workflow using prepared libraries. In this example, prepared libraries are used in a hybridization capture experiment prior to sequencing.
Use the tables to choose the right xGen library preparation kit for your research.
Table 1. Available IDT xGen DNA Library Prep Kits and their unique features.
| xGen DNA Library Prep Kit | xGen cfDNA & FFPE DNA Library Prep Kit | xGen ssDNA & Low-input DNA Library Prep Kit | |
|---|---|---|---|
| Key features | DNA library prep kit for high-quality samples (e.g., gDNA, metagenomics samples, high-quality FFPE) | High conversion library prep kit for low inputs or challenging samples (e.g., FFPE, cfDNA) | Library prep kit for low-quality degraded DNA, ssDNA, or mixtures of ssDNA and dsDNA |
| Workflow types | PCR-free + PCR-amplified | PCR-amplified | PCR-amplified |
| Fragmentation | Options for mechanical and enzymatic fragmentation | Mechanical | Mechanical |
| Input (ng) | Mechanical: 1 ng to 1 µg Enzymatic: 100 pg to 1 µg | 1 ng to 250 ng | 10 pg to 250 ng |
| Error correction options (UMIs) | Single read family with UDI-UMI adapters | Single read family & combined read family (UMIs built into the kit) | None |
Table 2. Available IDT xGen RNA Library Prep Kits and their unique features.
| xGen RNA Library Prep Kit | xGen Broad-Range RNA Library Prep Kit | |
|---|---|---|
| Key features | Fast workflow | Broad input range |
| Input | 5 ng to 100 ng (mRNA, rRNA-depleted RNA, total RNA) 100 ng to 500 ng (total RNA into library prep for hybridization capture) 100 ng to 1 µg (total RNA into an upstream module*) | 100 pg to 100 ng (mRNA, rRNA-depleted RNA, total RNA) 10 ng to 1 µg (total RNA into an upstream module*) |
| Sample type | Ribosomal RNA-depleted RNA Poly(A)-enriched mRNA Total RNA Formalin-fixed, paraffin-embedded (FFPE) RNA | Ribosomal RNA-depleted RNA Poly(A)-enriched mRNA Total RNA Formalin-fixed, paraffin-embedded (FFPE) RNA RNA with low DV200 scores |
| Time required** | ~3.5 hours | ~4.5 hours |
*Note the rRNA depletion and poly(A) enrichment modules are not included with this kit.
** These workflow times are based on standard methods to complete each task. Based upon internal laboratory procedures, results may vary.
Table 3. Characteristics of the xGen Methyl-Seq Library Prep Kit.
| xGen Methyl-Seq DNA Library Prep Kit | |
|---|---|
| Key features | Comprehensive coverage of the methylome for epigenetic research powered by xGen Adaptase technology* |
| Input DNA concentrations | 100 pg to 100 ng |
| Applications | Whole genome bisulfite sequencing (WGBS) Targeted bisulfite sequencing (with the use of an xGen Hybridization Capture Panel) Single-cell bisulfite sequencing Identifying genome-wide methylation of cfDNA in research studies |
| Time required** | ~2 hours |
*Note xGen Adaptase technology is a template independent adapter attachment which reduces workflow time while generating a less biased library.
** These workflow times are based on standard methods to complete each task. Based upon internal laboratory procedures, results may vary.
Ordering
Are you looking for guidance on product compatibility or how to select the appropriate library preparation kit? Investigate the options and see our recommended product groupings with our xGen NGS Solutions Builder Tool.
xGen NGS Solutions Builder Tool
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