CRISPR genome editing
CRISPR knock-out: Tips and tricks
For CRISPR gene knock-outs, it’s important to design the right guide RNA to increase genome editing efficiency. For a successful knock-out, the Cas nuclease makes a double strand break (DSB) which is repaired through the non-homologous end joining pathway (NHEJ). Read more about the tips and tricks you need to know to increase the efficiency of your CRISPR experiments.
- Prime editing with pegRNA: a novel and precise CRISPR genome editing system
- What is CRISPR screening?
- A mutant A.s. Cas12a (Cpf1) for CRISPR genome editing shows broad temperature tolerance and a high ratio of on:off target activity, with site-to-site reliability rivaling S.p. Cas9
- Single or two-part? Choosing the right gRNA format for CRISPR-Cas9 genome editing
- Using CRISPR-Cas9 technology for targeted nanopore sequencing
- High-fidelity Cas9 provides highly efficient genome editing with reduced off-target effects, even in primary cells
- When and how to use nickases for efficient genome editing
- Genome editing: Isolating clones for genotypic and phenotypic characterization
- Protocols for CRISPR genome editing in your model system
