Germline characterization with no concern for sample crosstalk
i.e., SNPs, indels, crude CNVs
UDI
SI, CDI, or UDI-UMI
Germline characterization where sample cross talk is important
i.e., translational research, inherited genetics, infectious disease
UDI
UDI-UMI
Low-level mutation detection, down to ~1% frequency
i.e., cancer, mosaicism, other diseases where somatic mutations are important
UDI-UMI
TruSeq™–Compatible Full-length Adapters
TruSeq™–Compatible Stubby Adapter
TruSeq™– or Nextera™–Compatible Indexing Primers
Delivered as
Duplex
Duplex
Forward and reverse primer mix
Normalized to
15 μM
15 μM
20 μM (10 μM each primer)
Volume delivered per duplex or primer pair
16 reaction
80 μl
80 μl
80 μl
96 reaction
480 μl
480 μl
480 μl
2 nmol
130 μl
130 μl
200 μl
8 nmol
530 μl
530 μl
800 μl
24 nmol
1600 μl
1600 μl
2400 μl
Suggested amount/reaction to use (may vary by application)
75 pmol in 5 μl
75 pmol in 5 μl
50 pmol (per primer) in 5 μl
Introduction
IDT understands the needs of genomics projects are not one-size-fits-all, and provides custom adapters that you can tailor to your needs. Our scientists have created this Custom Adapter Configurator tool to guide you through selection of several options as part of the adapter design process.
Consecutive, starting with index #1 and ending with index #
Consecutive, starting with index # and ending with index #
Processing
