The Iowa Black® (patent pending) quenchers are a new family of dark quenchers developed by IDT that are offered as either 5'- or 3'-end modifications. They have excellent properties for use in fluorescence quenched probes. These compounds are stable over a wide range of conditions, including pH and heat. Further, they provide a convenient hydrophobic handle which facilitates purification by HPLC methods.
Iowa Black® FQ has a broad absorbance spectra ranging from 420 to 620 nm with peak absorbance at 531 nm. This quencher is ideal for use with fluorescein and other fluorescent dyes that emit in the green to pink spectral range.
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Iowa Black® RQ has a broad absorbance spectra ranging from 500 to 700 nm with peak absorbance at 656 nm. This quencher is ideal for use with Texas Red®, Cy5TM, and other fluorescent dyes that emit in the red spectral range.
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