Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

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What cycling parameters will give me the best results when using the PrimeTime™ One-Step RT-qPCR Master Mix?

The master mix can be used with either standard or fast cycling conditions, depending on what qPCR system you are using. For best results, use one of the cycling parameters provided in the PrimeTime One-Step RT-qPCR Master Mix protocol:

  • Standard cycling conditions: 15 min. 50°C; 3 min. 95°C; 35–45x (15 sec. 95°C, 1 min. 60°C).
  • Fast cycling conditions: 15 min. 50°C; 3 min. 95°C; 35–45x (5 sec. 95°C, 30 sec. 60°C).

Categories:
qPCR & PCR

Tags:
master mix qpcr qpcr master mix