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The amount of fluorescently labeled Alt-R™ CRISPR-Cas9 tracrRNA [ATTO™ 550, ATTO 488, or ATTO 647 (ATTO-TEC, GmbH)] needed varies by application and transfection method. Here are our recommendations.
Use these as starting points as you optimize your CRISPR-Cas9 experiments.
| FACS analysis | Fluorescence microscopy | |
|---|---|---|
| Lipofection | ~10 nM | 10 nM |
| Electroporation | ≥0.5 µM |
~0.5 µM |