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LIQUIDPlex assays, powered by Anchored Multiplex PCR (AMP™) chemistry, preferentially amplify low molecular weight cfDNA fragments. Adapters are ligated to end-repaired input cfDNA fragments prior to amplification with a gene specific primer and a universal primer at the adapter region. High molecular weight genomic DNA (gDNA) fragments in the input sample are too long for complete amplification, allowing for optimal amplification of cfDNA with LIQUIDPlex assays.