Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
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How do I sequence the UMI in xGen™ UDI-UMI Adapters?
The UMI (dark blue in the illustration below) is positioned next to the i7 index (light orange) so that it will be sequenced as part of the i7 index read.
So, to sequence the UMI, increase the number of cycles for the i7 index read by 9 cycles. Refer to the IDT Master Index List for guidelines concerning the xGen™ UDI-UMI adapters.
