Obtain high quality qPCR results using PrimeTime™ Gene Expression Master Mix

Reverse transcription quantitative PCR (RT-qPCR) is the method of choice for accurate and fast gene expression analysis. With more and more researchers relying on PrimeTime qPCR Probes and custom assays, we recognized the need for a quality gene expression master mix to accompany these products.

PrimeTime Gene Expression Master Mix is a versatile and convenient master mix for 5′ nuclease assays. It has been designed for qPCR and 2-step RT-qPCR using PrimeTime qPCR Probe Assays, but also can be used with primer-probe sets from IDT and other manufacturers. In addition, this master mix is compatible with a wide range of instruments—whether you prefer standard or fast cycling conditions, and whether or not you need a reference dye.

Useful for high-throughput or overnight applications

PrimeTime Gene Expression Master Mix has been formulated for use at room temperature, so you can let your reactions cycle overnight or perform automated, high-throughput experiments (Figure 1). C_q values were consistent when qPCRs were run immediately or after 24 hr at room temperature. The standard deviations for the C_q values were <0.5 for template levels >10 copies. This result was obtained with 3 different lots of master mix, so you can rely on this reagent to provide reliable results when used in your qPCR assays.

Figure 1. Consistent results at 0 and 24 hours across different lots of PrimeTime Gene Expression Master Mix. qPCR mixes of PrimeTime HPRT qPCR Assay (Assay ID: Hs.PT.58v.45621572), PrimeTime Gene Expression Master Mix (from 1 of 3 lots), reference dye, and varying amounts of gBlocks™ Gene Fragments (10–10⁷ copies; 8 replicates) were assembled. A JANUS^® automated, liquid-handling workstation (PerkinElmer) was used to load the reactions into PCR plates. Reactions were run immediately (Day 0) or after 24 hours (Day 1) on a QuantStudio™ 7 Flex System (Thermo Fisher Scientific).

For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations. RUO22-1577_001